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Probability Assignment

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MS/MS Interpretation

ProbID

Please note; this documentation may be out of date. Please see the ProbID documentation on the SPCTools Wiki for up-to-date information.

Overview
ProbID is a software tool designed to identify peptides from tandem mass spectra using a protein sequence database. It computes z scores for the identifications and output the results in pepXML format, which subsequently can be analyzed by TPP (trans-proteomic pipeline).

Components of downloadable package:

Installation unzip ProbID.tgz (tar -xzf ProbID.tgz)

Running ProbID

Both options, your have to have probid.param in the same directory where your data locate.